Flies underwent optogenetic activation in mushroom body neurons prior to lysate extraction and tandem purification.

Source was adult heads of wild-type fly line; bait produced from transgenic construct; prey produced from endogenous gene.

Phosphatase treatment reduces Kdm4B-bur interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus system; prey froduced from transgenic construct.

Source was adult heads of transgenic fly line; bait and prey produced from tagged transgenic constructs.

NOS inhibition and knockdown of either Gycβ100B or Pkg21D, disrupts Kdm4B-bur interaction. 8-Bromo-cGMP feeding increased interaction.