FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
M6, bark
FlyBase ID
FBig0000123160
Interaction Network
Interactions Browser links
M6 network
bark network
Reported Interactions
FBrf0259086-1.coIP.WB
Reference
Schleutker and Luschnig, 2024
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
M6 

M6

bait

GFP tag

M6 
bark 

Aka

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
M6 
Palmitoylated cysteine residues
mutation decreasing interaction
aa 76,77 and 80

C76S, C77S, C80S

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Schleutker and Luschnig, 2024)
FBrf0259086-3.coIP.WB
Reference
Schleutker and Luschnig, 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
M6 

M6

bait

GFP tag

bark 

Aka

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

All M6 isoforms B-F interact with bark.

(Schleutker and Luschnig, 2024)

Source was cell extract of S2R_ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schleutker and Luschnig, 2024)
FBrf0259086-5.coIP.WB
Reference
Schleutker and Luschnig, 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
M6 

M6

bait

GFP tag

bark 

Aka

prey
bark 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bark 
CLTD and TM domains
sufficient binding region
aa 76,77 and 80

C76S, C77S, C80S

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R_ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Schleutker and Luschnig, 2024)
External Crossreferences and Linkouts ( 0 )
References (1)