FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000123205
Interaction Network
Interactions Browser links
N network
zip network
Reported Interactions
FBrf0259327-1.coIP.MS
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait
N 
zip 

Zip

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
unspecified

coordinates relative to N-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tissue lysate was prepared from hyperplastic wing imaginal discs over-expressing N-ICD.

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

FBrf0259327-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait
zip 

Zip

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head tissues of transgenic fly line; bait produced from transgenic construct; prey produced from tagged transgenic construct.

Tissue lysate was prepared from adult head tissues over-expressing full-length N and GFP-zip.

FBrf0259327-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait
zip 

Zip

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tissue lysate was prepared from adult head tissues over-expressing full-length N and GFP-zip. Endogenous zip and GFP-zip were recognized by zip antibody as a double band in the western blot.

Source was adult head tissues of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

FBrf0259327-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
zip 

Zip

bait

GFP tag

N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head tissues of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

Tissue lysate was prepared from adult head tissues over-expressing full-length N and GFP-zip.

External Crossreferences and Linkouts ( 0 )
References (1)