FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Diap1, rdx
FlyBase ID
FBig0000123241
Interaction Network
Interactions Browser links
Diap1 network
rdx network
Reported Interactions
FBrf0259668-1.coIP.WB
Reference
Liu et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rdx 

rdx

bait

FLAG tag

Diap1 

Diap1

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2021)
FBrf0259668-2.coIP.WB
Reference
Liu et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Diap1 

Diap1

bait

FLAG tag

Diap1 
Diap1 
rdx 

rdx

prey

Myc tag

rdx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rdx 
MATH domain
sufficient binding region
aa 1-179

coordinates relative to rdx-PB

Diap1 
SPOP binding consensus (SBC) 1
mutation decreasing interaction
aa 316-320

316EESSS320 to AAAAA; coordinates relative to Diap1-PA

Diap1 
SPOP binding consensus (SBC) 2
mutation decreasing interaction
aa 380-384

380SGSTS384 to AAAAA; coordinates relative to Diap1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2021)
FBrf0259668-3.coIP.WB
Reference
Liu et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rdx 

rdx

bait

Myc tag

rdx 
Diap1 

Diap1

prey

FLAG tag

Diap1 
Diap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rdx 
MATH domain
sufficient binding region
aa 1-179

coordinates relative to rdx-PB

Diap1 
SPOP binding consensus (SBC) 1
mutation decreasing interaction
aa 316-320

316EESSS320 to AAAAA; coordinates relative to Diap1-PA

Diap1 
SPOP binding consensus (SBC) 2
mutation decreasing interaction
aa 380-384

380SGSTS384 to AAAAA; coordinates relative to Diap1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)