FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Usp14, cactin
FlyBase ID
FBig0000123262
Interaction Network
Interactions Browser links
Usp14 network
cactin network
Reported Interactions
FBrf0259522-1.coIP.WB
Reference
Rong et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Usp14 

Usp14

bait

FLAG tag

cactin 

cactin

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rong et al., 2024)
FBrf0259522-2.coIP.WB
Reference
Rong et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
cactin 

cactin

bait

V5 tag

cactin 
Usp14 

Usp14

prey

FLAG tag

Usp14 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Usp14 
USP domain
necessary binding region
aa 105-476

coordinates relative to Usp14-PA

cactin 
C-Cactus domain
necessary binding region
aa 595-719

coordinates relative to cactin-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rong et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)