FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
foxo
FlyBase ID
FBig0000123333
Interaction Network
Interactions Browser links
foxo network
Reported Interactions
FBrf0260347-12.coIP.WB
Reference
Sun et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
foxo 

Foxo

bait

HA tag

foxo 

Foxo

prey

FLAG tag

foxo 
foxo 
foxo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
foxo 
C-1-terminal domain
sufficient binding region
aa 412-622

coordinates relative to foxo-PF or foxo-PG

foxo 
N-terminal domain
sufficient binding region
aa 1-311

coordinates relative to foxo-PF or foxo-PG

foxo 
ubiquitination residues 182 and 205
mutation decreasing interaction
aa 182 and 205

K182R, K205R; within the context of aa 1-311; coordinates relative to foxo-PF or foxo-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2024)

Addition of hydrogen peroxide diminished foxo-foxo interaction. foxo N-terminal ubiquitination residue mutations diminishes its interaction with C-terminal foxo (aa 312-622)

(Sun et al., 2024)
FBrf0260347-13.coIP.WB
Reference
Sun et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
foxo 

Foxo

bait

FLAG tag

foxo 

Foxo

prey

HA tag

foxo 
foxo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
foxo 
C-1-terminal domain
sufficient binding region
aa 412-622

coordinates relative to foxo-PF or foxo-PG

foxo 
N-terminal domain
sufficient binding region
aa 1-311

coordinates relative to foxo-PF or foxo-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

foxo C-terminal domain coimmunoprecipitates foxo N-terminal domain, but not its own C-terminal domain. Addition of hydrogen peroxide diminishes foxo-foxo interaction.

(Sun et al., 2024)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)