FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
t-Grip91, Sas-4
FlyBase ID
FBig0000123367
Interaction Network
Interactions Browser links
t-Grip91 network
Sas-4 network
Reported Interactions
FBrf0260790-1.Y2H
Reference
Alzyoud et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas4

bait

fused to GAL4 DNA-binding domain

t-Grip91 

t-Grip91

prey

fused to GAL4 activation domain

t-Grip91 
t-Grip91 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
t-Grip91 
N-terminal domain
sufficient binding region
aa 1-966

coordinates relative to t-Grip91-PA

t-Grip91 
C-terminal domain
sufficient binding region
aa 1-966

coordinates relative to t-Grip91-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alzyoud et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Alzyoud et al., 2024)
FBrf0260790-10.PD.A
Reference
Alzyoud et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
t-Grip91 

t-Grip91

bait

GST tag

t-Grip91 
t-Grip91 
Sas-4 

Sas4

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
t-Grip91 
N-terminal domain
sufficient binding region
aa 1-966

coordinates relative to t-Grip91-PA

t-Grip91 
C-terminal domain
sufficient binding region
aa 967-1932

coordinates relative to t-Grip91-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Alzyoud et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)