FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rac1, cyri
FlyBase ID
FBig0000123389
Interaction Network
Interactions Browser links
Rac1 network
cyri network
Reported Interactions
FBrf0260768-1.PD.WB
Reference
Rötte et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
cyri 

CYRI

bait

GST tag

cyri 
Rac1 

Rac1

prey

HA tag

Rac1 
Rac1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cyri 
interaction domain residues 163 and 164
mutation decreasing interaction
aa 163-164

R163D, R164D

Rac1 
constitutively residue 12
mutation increasing interaction
aa 12

G12V

Rac1 
dominant negative residue
mutation decreasing interaction
aa 17

T17N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Reactions were preloaded with GDP or GTPγS. cyri interacts preferentially with activated Rac1.

(Rötte et al., 2024)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct.

(Rötte et al., 2024)
FBrf0260768-2.PD.WB
Reference
Rötte et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Rac1 

Rac1

bait

GST tag

Rac1 
cyri 

CYRI

prey

HA tag

cyri 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cyri 
interaction domain residues 163 and 164
mutation decreasing interaction
aa 163-164

R163D, R164D

Rac1 
constitutively residue 61
mutation increasing interaction
aa 61

Q61L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Reactions were preloaded with GDP or GTPγS. cyri interacts preferentially with activated Rac1.

(Rötte et al., 2024)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct.

(Rötte et al., 2024)
FBrf0260768-3.BiFC.FM
Reference
Rötte et al., 2024
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rac1 

Rac1

bait

YFP tag, N-terminal fragment

cyri 

CYRI

prey

YFP tag, C-terminal fragment

cyri 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cyri 
interaction domain residues 163 and 164
mutation decreasing interaction
aa 163-164

R163D, R164D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait and prey produced from transgenic constructs.

(Rötte et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)