lncRNA:DREAMer is immunoprecipitated by all proteins of the DREAMer complex when they are in solution together (Fig6C), however when E2f2 is removed, lncRNA:DREAMer is not immunoprecipitated (Fig6E), indicating lncRNA:DREAMer interacts with the DREAMer complex via a direct interac-
tion with E2f2.
Interaction in vitro; bait prey produced by in vitro translation; prey produced by in vitro transcription.
Biotinylated lncRNA:DREAMer pulls down all proteins of the DREAMer complex when they are in solution together, however when E2f2 is removed, lncRNA:DREAMer is not able to pull down DREAMer complex proteins, indicating lncRNA:DREAMer interacts with the DREAMer complex via a direct interac-
tion with E2f2.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.
Source was larval salivary gland of wild-type fly line; bait and prey produced from endogenous gene.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey prey derived from wild-type larval salivary glands.
