FB2021_01, released Feb 18, 2021

Gene: Dmel\mip130

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General Information
Symbol
Dmel\mip130
Species
D. melanogaster
Name
Myb-interacting protein 130
Annotation Symbol
CG3480
Feature Type
protein_coding_gene
FlyBase ID
FBgn0023509
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Snapshot
Myb-interacting protein 130 (mip130) encodes a subunit of the MuvB core complex. The MuvB core binds to the oncoprotein encoded by Myb and the Rbf-E2f2-Dp tumor suppressor complex, thereby controlling the expression of many genes, including critical regulators of the cell cycle. [Date last reviewed: 2019-03-21]
Other Summaries
Also Known As

twit, EG:86E4.4 , twilight

Key Links
Genomic Location
Cytogenetic map
2B15-2B16
Sequence location
X:1,908,239..1,920,318 [+]
Recombination map

1-0.2

RefSeq locus
NC_004354 REGION:1908239..1920318
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables DNA binding
inferred from direct assay
(Beall et al., 2002)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000495591
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in centrosome cycle
inferred from mutant phenotype
(Goshima et al., 2007)
involved_in negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Korenjak et al., 2004, Lewis et al., 2004)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in eggshell chorion gene amplification
inferred by curator from GO:0031523
(Beall et al., 2002)
involved_in regulation of cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000495591
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000495591
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in reproduction
inferred from biological aspect of ancestor with PANTHER:PTN000495591
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Beall et al., 2002)
located_in nucleus
inferred from high throughput direct assay
(Lye et al., 2014)
located_in polytene chromosome
inferred from direct assay
(Beall et al., 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in nucleoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000495591
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in nucleus
inferred from sequence or structural similarity
(White-Cooper et al., 2000)
part_of transcription repressor complex
inferred from electronic annotation with InterPro:IPR010561
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mip130 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mip130-RA
FBtr0070315
NM_130602
3459
986
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
mip130-PA
FBpp0070302
110.4
986
4.93
NP_569958
AAF45694
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mip130 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
• membrane
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Beall et al., 2004)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Beall et al., 2002)
located_in nucleus
inferred from high throughput direct assay
(Lye et al., 2014)
located_in polytene chromosome
inferred from direct assay
(Beall et al., 2007)
Expression Deduced from Reporters
Reporter: PBac{754.P.FSVS-0}mip130CPTI003151
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mip130 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mip130
Transgenic constructs containing regulatory region of mip130
Aberrations (Deficiencies and Duplications) ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\LIN9
12 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Lin9
11 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Lin9
8 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\lin9
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\lin9
13 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\lin-9
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (3)
Atha\ALY3
3 of 9
Yes
Yes
Atha\ALY1
2 of 9
No
Yes
Atha\ALY2
2 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919032H )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005170
Drosophila simulans
Dsim\GD24729
Drosophila sechellia
Dsec\GM19180
Drosophila erecta
Dere\GG12891
Drosophila yakuba
Dyak\GE16223
Drosophila ananassae
Dana\GF19452
Drosophila pseudoobscura pseudoobscura
Dpse\GA27784
Drosophila persimilis
Dper\GL18218
Drosophila willistoni
Dwil\GK16330
Drosophila virilis
Dvir\GJ16785
Drosophila mojavensis
Dmoj\GI14720
Drosophila grimshawi
Dgri\GH11979
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503PP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024269
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10271
Mayetiola destructor
Hessian fly
Mdes\Mdes007278
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005631
Anopheles darlingi
American malaria mosquito
Adar\ADAC007010
Anopheles gambiae
Malaria mosquito
Agam\AGAP004624
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ012736
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03I4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA001154
Danaus plexippus
Monarch butterfly
Dple\DPOGS211921-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL006845
Apis florea
Little honeybee
Aflo\2825983364
Apis mellifera
Western honey bee
Amel\LOC410863
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17018
Bombus terrestris
Buff-tailed bumblebee
Bter\15214978
Linepithema humile
Argentine ant
Lhum\LH19032
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000e65
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009488
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04529
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04203
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00738
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_08121
Pediculus humanus
Human body louse
Phum\PHUM614730
Rhodnius prolixus
Kissing bug
Rpro\RPRC009269
Cimex lectularius
Bed bug
Clec\CLEC002528
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005224
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_10033
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03EV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR006059
Ixodes scapularis
Black-legged tick
Isca\ISCW005548
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62297.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur10g03350
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_300555
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G049W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_008516gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Lin9
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_008517gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000023003
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000009233
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
aly
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    LIN9; lin-9 DREAM MuvB core complex component
    12 of 15
    609375
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        mip130 - CTCF
        anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, western blot
        (Bohla et al., 2014)
        mip130 - Caf1-55
        anti bait coimmunoprecipitation, western blot
        (Lewis et al., 2004, Beall et al., 2002)
        mip130 - Cp190
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
        (Bohla et al., 2014, Korenjak et al., 2014)
        mip130 - Dp
        anti bait coimmunoprecipitation, western blot
        (Lewis et al., 2004)
        mip130 - E2f2
        anti bait coimmunoprecipitation, western blot
        (Lewis et al., 2004)
        mip130 - Myb
        isothermal titration calorimetry, predetermined participant, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
        (Vorster et al., 2020, Andrejka et al., 2011, Wen et al., 2008, Beall et al., 2007, Lewis et al., 2004, Beall et al., 2002)
        mip130 - Rbf
        anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot
        (Korenjak et al., 2004, Lewis et al., 2004)
        mip130 - Rbf2
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot
        (Korenjak et al., 2004, Lewis et al., 2004)
        mip130 - lin-52
        anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular sieving
        (Lewis et al., 2012, Lewis et al., 2004)
        mip130 - mip120
        anti bait coimmunoprecipitation, western blot
        (Lewis et al., 2004, Beall et al., 2002)
        mip130 - mip40
        anti bait coimmunoprecipitation, western blot, affinity chromatography technology, Identification by mass spectrometry
        (Beall et al., 2007, Lewis et al., 2004, Beall et al., 2002)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        lin-52
        suppressible
        mip130
        (Lewis et al., 2012)
        Myb
        suppressible
        mip130
        (Beall et al., 2004)
        Myb|lin-52
        suppressible
        mip130
        (Lewis et al., 2012)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-0.2

        Cytogenetic map
        2B15-2B16
        Sequence location
        X:1,908,239..1,920,318 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        2B15-2B16
        Limits computationally determined from genome sequence between P{EP}EP1444&P{EP}CG14818EP1190 and P{EP}CG3600EP1232
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        1-0.2

        (FlyBase, 2016, Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (13)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (75)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments

        "l(1)G0220" may affect "CG17766" or "twit".

        (Peter et al., 2002)
        Other Comments

        RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of monastral bipolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

        (Goshima et al., 2007)

        RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells does not affect the number of prophase centrosomes, but monastral bipolar spindles are formed during mitosis, suggesting that the centrosomes are fused.

        (Goshima et al., 2007)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 36 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (12)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (74)