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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000089405
Interaction Network
Interactions Browser links
Myb network
mip130 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0155469-10.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

p130

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Identical results were obtained with ovary nuclear extract. IPs were either performed with or without ethidium bromide in the extracts before precipitation to demonstrate that DNA was unnecessary for interaction.

FBrf0155469-18.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

p130

bait
Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Identical results were obtained with ovary nuclear extract. IPs were either performed with or without ethidium bromide in the extracts before precipitation to demonstrate that DNA was unnecessary for interaction.

FBrf0180259-19.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

Mip130

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.

FBrf0180259-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Mip130

bait
Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

All immunoprecipitations were performed in the presence of ethidium bromide in order to disrupt protein DNA interactions.

FBrf0200827-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Mip130

bait
Myb 

Myb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testis of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0202994-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

FLAG tag

Myb 

Mip130

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myb 
C-terminal domain
sufficient binding region
unspecified

C-terminal fragment of Myb lacking the DNA binding domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0216444-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myb 

Myb

bait

FLAG tag

Myb 

Mip130

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myb 
C terminus
unspecified role

conserved animal-specific C-terminal domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0245601-1.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Mip130

bait
Myb 

dMyb

prey
Myb 
Myb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myb 
interaction domain
mutation decreasing interaction
aa 606,607

K606A,W607A

Myb 
interaction domain
mutation disrupting interaction
aa 617,618

D617A,Q618A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding of Myb to a mip130-lin-52 heterodimer was captured as two binary interactions.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)