Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Addition of hh inhibited ci-tai interaction.

tai inhibits ci DNA binding.

tai and ci were incubated simultaneously with biotin labeled DNA and detected by chemiluminescence. Diminishment of ci DNA binding by addition of tai was used to deduce protein-protein interaction. Addition of tai after pre-incubation of ci and DNA did not diminish ci DNA binding suggesting that tai cannot dissociate

the existing ci-DNA complex.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.