FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Brat1, IntS11
FlyBase ID
FBig0000123411
Interaction Network
Interactions Browser links
Brat1 network
IntS11 network
Reported Interactions
FBrf0260214-15.coIP.WB
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Brat1 

CG7044

bait

FLAG tag

IntS11 

IntS11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-30.coIP.MS
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Brat1 

CG7044

bait

FLAG tag

IntS11 

IntS11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-35.coIP.WB
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

Brat1 

CG7044

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-40.coIP.MS
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

Brat1 

CG7044

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-45.PD.CH.MW.EM
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

(Lin et al., 2024)
FBrf0260214-47.PD.CH.MW
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Brat1 

CG7044

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

(Lin et al., 2024)

IntS11, IntS9 and Brat1 form a heterotrimer in vitro. Captured as three pairwise interactions.

(Lin et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)