Raf
Pyo tag
HYP
GST tag
Co-expression of cnk-ave enhances Raf-Dsor1 interaction, which is abrogated by ksr M902D or cnk D195K mutation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
L90D, I97D; coordinates relative to ave-PA
Interaction occurred with co-expression of Ras85D, Dsor1 and ksr. Mutations in either ave or cnk that destabilize the ave-cnk scaffolding complex, decrease its ability to pull down Raf. Mutations in ksr reduce ave\'s ability to pull down Raf.
