bbn, Mycbp Physical Interaction report

Identification by mass spectrometry

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bbn

bait

HA tag

Mycbp

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from endogenously tagged gene; prey produced from endogenous gene.

(Mercer et al., 2025)

FBrf0262100-5.coIP.WB

Reference

Mercer et al., 2025

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bbn

bait

HA tag

Mycbp

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Serial coIP experiments indicate that CG14545, CG17202, and otu proteins associate as a trimeric complex. Captured as three pairwise interactions.

(Mercer et al., 2025)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mercer et al., 2025)

FBrf0263985-2.coIP.WB

Reference

Azlan and Fukunaga, 2025

Description

physical association

Assay

anti bait coimmunoprecipitation

western blot

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mycbp

bait

EGFP tag

bbn

Sakura

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Azlan and Fukunaga, 2025)

FBrf0263985-4.coIP.WB

Reference

Azlan and Fukunaga, 2025

Description

physical association

Assay

western blot

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mycbp

bait

EGFP tag

bbn

Sakura

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovaries of wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Azlan and Fukunaga, 2025)

FBrf0263985-6.coIP.WB

Reference

Azlan and Fukunaga, 2025

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Mycbp

bait

HA tag

bbn

Sakura

prey

FLAG tag

bbn

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

bbn

interaction domain

sufficient binding region

aa 1-100

coordinates relative to bbn-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Azlan and Fukunaga, 2025)

FBrf0263985-7.coIP.WB

Reference

Azlan and Fukunaga, 2025

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bbn

Sakura

bait

EGFP-FLAG tag

Mycbp

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

bbn, Mycbp and otu form a complex demonstrated by sequential coimmunoprecipitation. Captured in pairwise interactions.

(Azlan and Fukunaga, 2025)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Azlan and Fukunaga, 2025)

FBrf0263985-9.coIP.WB

Reference

Azlan and Fukunaga, 2025

Description

physical association

Assay

Collection

Source/Stage

Cell line used