Ubx forms large fibrils in vitro, visible by scanning electron microscopy.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
Purified recombinant Ubx was incubated in a Langmuir trough for varying amounts of time, aggregates were then pulled onto a film by the Langmuir technique to form larger fibrils.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
