FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
meru, mud
FlyBase ID
FBig0000123771
Interaction Network
Interactions Browser links
meru network
mud network
Reported Interactions
FBrf0262387-1.coIP.WB
Reference
McLellan et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mud 

Mud

bait

Myc tag

mud 
meru 

Meru

prey

FLAG tag

meru 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mud 
interaction domain
sufficient binding region
aa 1825-2456

coordinates relative to mud-PH

meru 
RxL motif
necessary binding region
aa 258-261

LLHL to AAAA; coordinates relative to meru-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLellan et al., 2025)
FBrf0262387-10.coIP.WB
Reference
McLellan et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mud 

Mud

bait

GFP tag

mud 
meru 

Meru

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mud 
interaction domain
sufficient binding region
aa 1452-1961

coordinates relative to mud-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLellan et al., 2025)
FBrf0262387-6.coIP.WB
Reference
McLellan et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
meru 

Meru

bait

GFP tag

meru 
meru 
mud 

Mud

prey

FLAG tag

mud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
meru 
RxL motif
necessary binding region
aa 258-261

LLHL to AAAA; coordinates relative to meru-PA

meru 
Phosphorylation residues
mutation decreasing interaction
aa 27, 60, 67, 274, 496

S27A, S60A, T267A, T274A, and S496A; coordinates relative to meru-PA

mud 
interaction domain
sufficient binding region
aa 1452-1961

coordinates relative to mud-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

RNAi of CycA reduced meru-mud interaction.

(McLellan et al., 2025)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLellan et al., 2025)
FBrf0262387-9.coIP.WB
Reference
McLellan et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mud 

Mud

bait

GFP tag

meru 

Meru

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLellan et al., 2025)

Co-expression of Cdk1 and CycA increases mud-meru interaction.

(McLellan et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)