FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tox4, PNUTS
FlyBase ID
FBig0000123858
Interaction Network
Interactions Browser links
Tox4 network
PNUTS network
Reported Interactions
FBrf0262508-1.Y2H
Reference
Duncalf et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PNUTS 

PNUTS

bait

fused to LexA DNA-binding domain

Tox4 

Tox4

prey

fused to GAL4 activation domain

Tox4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tox4 
C-terminal domain
sufficient binding region
aa 206-246

coordinates relative to Tox4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Duncalf et al., 2025)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Duncalf et al., 2025)
FBrf0262508-2.coIP.WB
Reference
Duncalf et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Tox4 

Tox4

bait

GFP tag

Tox4 
PNUTS 

PNUTS

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tox4 
C-terminal domain
necessary binding region
aa 216-250

coordinates relative to Tox4-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Duncalf et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)