FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tmem43, porin
FlyBase ID
FBig0000123979
Interaction Network
Interactions Browser links
Tmem43 network
porin network
Reported Interactions
FBrf0263857-1.coIP.WB
Reference
Jürgens et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tmem43 

Tmem43

bait

GFP tag

Tmem43 
porin 

porin

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tmem43 
interaction domain residue 333
mutation decreasing interaction
aa 333

S333L; coordinates relative to Tmem43-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jürgens et al., 2025)
FBrf0263857-2.coIP.MS
Reference
Jürgens et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tmem43 

Tmem43

bait

HA tag

Tmem43 
porin 

porin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tmem43 
interaction domain residue 333
mutation disrupting interaction
aa 333

S333L; coordinates relative to Tmem43-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was third instar larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jürgens et al., 2025)
FBrf0263857-3.PL.MS
Reference
Jürgens et al., 2025
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tmem43 

Tmem43

bait

TurboID tag

Tmem43 
porin 

porin

prey

Biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tmem43 
interaction domain residue 333
mutation decreasing interaction
aa 333

S333L; coordinates relative to Tmem43-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was third instar larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Jürgens et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)