FlyBase Gene Report: Dmel\porin

General Information

Symbol

Dmel\porin

Species

D. melanogaster

Name

porin

Annotation Symbol

CG6647

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004363

Gene Model Status

Stock Availability

12 publicly available

Gene Summary

porin (porin) encodes a beta-barrel channel protein localized in the outer membrane of mitochondria that conducts translocation of various ions and metabolites between cytoplasm and mitochondria. It plays roles in mitochondria dynamics and mitophagy, and is ubiquitinated by the E3 ligases encoded by park and Mul1. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

VDAC, l(2)k05123, DmVDAC, POR-1, VDAC1

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:10,847,227..10,850,848 [-]

Recombination map

2-43

RefSeq locus

NT_033779 REGION:10847227..10850848

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables voltage-gated anion channel activity

inferred from direct assay

(Komarov et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables voltage-gated anion channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000201897

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in ion transport

inferred from direct assay

(Komarov et al., 2004)

involved_in mitochondrion organization

inferred from mutant phenotype

(Park et al., 2010)

involved_in negative regulation of apoptotic process

inferred from mutant phenotype

(assigned by UniProt )

(Ham et al., 2020)

involved_in negative regulation of calcium import into the mitochondrion

inferred from mutant phenotype

(assigned by UniProt )

(Ham et al., 2020)

involved_in phototransduction

inferred from mutant phenotype

(Lee et al., 2007)

involved_in sperm individualization

inferred from mutant phenotype

(Park et al., 2010)

involved_in sperm mitochondrion organization

inferred from mutant phenotype

(Park et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in anion transmembrane transport

inferred from electronic annotation with InterPro:IPR001925

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

located_in Nebenkern

inferred from direct assay

(Oliva et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in mitochondrial outer membrane

inferred from biological aspect of ancestor with PANTHER:PTN000201897

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the eukaryotic mitochondrial porin family. (Q94920)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

porin (porin) encodes a beta-barrel channel protein localized in the outer membrane of mitochondria that conducts translocation of various ions and metabolites between cytoplasm and mitochondria. It plays roles in mitochondria dynamics and mitophagy, and is ubiquitinated by the E3 ligases encoded by park and Mul1. [Date last reviewed: 2019-03-14]

Protein Function (UniProtKB)

Forms a channel through the mitochondrial outer membrane and also the plasma membrane (By similarity). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (By similarity). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). In depolarized mitochondria, acts downstream of park to promote mitophagy or prevent apoptosis; polyubiquitination by park promotes mitophagy, while monoubiquitination by park decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033).

(UniProt, Q94920)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

5

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\porin for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0080182

1097

282

FBtr0080183

1614

282

FBtr0100584

2625

282

FBtr0305260

1525

282

FBtr0332029

1387

282

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0079771

30.5

282

6.97

FBpp0079772

30.5

282

6.97

FBpp0100039

30.5

282

6.97

FBpp0293784

30.5

282

6.97

FBpp0304343

30.5

282

6.97

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

282 aa isoforms: porin-PA, porin-PB, porin-PC, porin-PD, porin-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

282 (aa)

(Caggese et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with hexokinases.

(UniProt, Q94920)

Post Translational Modification

Ubiquitinated (PubMed:32047033). Undergoes monoubiquitination and polyubiquitination by park; monoubiquitination at Lys-273 inhibits apoptosis, whereas polyubiquitination at Lys-11, Lys-19, Lys-52 and Lys-109 may promote mitophagy (PubMed:32047033).

(UniProt, Q94920)

Domain

Consists mainly of membrane-spanning sided beta-sheets.

(UniProt, Q94920)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\porin using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

anterior endoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

(Fisher et al., 2012)

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

longitudinal visceral muscle primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval visceral muscle

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

oogenesis stage S9 -- S10

centripetal follicle cell

(Manning et al., 2017)

egg chamber | restricted

(Manning et al., 2017)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Specchia et al., 2008)

adult stage | female

(Specchia et al., 2008)

Additional Descriptive Data

porin transcripts are abundant in both testis and ovary

(Specchia et al., 2008)

Three transcript sizes observed on Northern Blot. Sizes were not reported.

(Oliva et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Oliva et al., 2002)

• Nebenkern

(Oliva et al., 2002)

(Specchia et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage -- adult stage

(Oliva et al., 2002)

adult stage | male

(Specchia et al., 2008)

adult stage | female

(Specchia et al., 2008)

Additional Descriptive Data

porin protein is abundant in both testis and ovary. In ovary it is observed in the follicle cells.

(Specchia et al., 2008)

Expression of porin is exclusively concentrated in the Nebenkern bodies of spermatids.

(Oliva et al., 2002)

Marker for

(Oliva et al., 2002)

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

located_in Nebenkern

inferred from direct assay

(Oliva et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\porin in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\porin

View exonic expression by tissue for Dmel\porin

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004363

DRscDB

34500/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0004363

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6647-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6647

Flygut - An atlas of the Drosophila adult midgut

FBgn0004363

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\porin

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 30 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of porin

Transgenic constructs containing regulatory region of porin

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene not disrupted in

(Spradling et al., 1999)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene disrupted in

(Christensen and Cook, 2006.12.5)

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

porin^KK107645

lethal (with porin^1rev6)

lethal (with porin^1rev17)

lethal (with porin^1rev23)

lethal (with porin^2rev8)

lethal (with porin^k05123)

lethal (with porin^k08405)

lethal | recessive

partially lethal - majority die | recessive

porin^Rev8

Sterility

Allele

female semi-sterile | recessive

porin^Rev8

porin^f03616

male fertile (with porin^1rev2)

male fertile (with porin^1rev16)

male fertile (with porin^1rev18)

male fertile (with porin^1rev21)

male fertile (with porin^2rev2)

male fertile (with porin^2rev3)

male fertile (with porin^2rev7)

male fertile (with porin^2rev14)

male fertile (with porin^k05123)

male fertile (with porin^k08405)

male semi-fertile

porin^EY02333

porin^A2

male sterile (with porin^2rev9)

male sterile (with porin^k05123)

porin^2rev9

male sterile (with porin^k08405)

porin^2rev9

male sterile | recessive

porin^Rev8

Other Phenotypes

Allele

abnormal flight

porin^A2

abnormal locomotor behavior

porin^Rev8

abnormal locomotor behavior (with porin^k05123)

porin^k08405

abnormal locomotor behavior (with porin^k08405)

porin^k05123

abnormal locomotor behavior | adult stage

porin^A2

abnormal locomotor behavior | recessive

abnormal neurophysiology | adult stage | somatic clone

porin^k05123

abnormal neurophysiology | larval stage

porin^Rev8

abnormal neurophysiology | recessive

porin^G2294

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

porin^JF03251

decreased fecundity | female | recessive

porin^Rev8

increased cell death | adult stage | progressive | somatic clone

porin^k05123

porin^Rev8

short lived (with porin^k05123)

porin^k08405

short lived (with porin^k08405)

porin^k05123

short lived | recessive

some die during larval stage | recessive

porin^Rev8

Phenotype manifest in

Allele

porin^A2

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

porin^JF03251

embryonic/larval neuromuscular junction

porin^Rev8

eye | somatic clone

porin^k05123

eye photoreceptor cell | somatic clone

porin^k05123

indirect flight muscle

indirect flight muscle, with Scer\GAL4^Mef2.PR

indirect flight muscle (with Df(2L)BSC143)

porin^A2

mitochondrion, with Scer\GAL4^Mef2.PR

mitochondrion (with Df(2L)BSC143)

porin^A2

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

porin^JF03251

porin^A2

porin^G2294

retina | somatic clone

porin^k05123

rhabdomere of eye photoreceptor cell | somatic clone

porin^k05123

seminal vesicle

porin^Rev8

porin^Rev8

porin^A2

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

14 of 15

Yes

Yes

13 of 15

No

Yes

2

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

13 of 15

No

Yes

11 of 15

No

Yes

Rattus norvegicus (Norway rat) (3)

13 of 13

Yes

Yes

12 of 13

No

Yes

9 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (3)

12 of 12

Yes

Yes

9 of 12

No

Yes

8 of 12

No

Yes

Danio rerio (Zebrafish) (4)

14 of 15

Yes

Yes

13 of 15

No

Yes

13 of 15

No

Yes

12 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

14 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (6)

8 of 9

Yes

Yes

8 of 9

Yes

Yes

8 of 9

Yes

Yes

7 of 9

No

Yes

5 of 9

No

Yes

3 of 9

No

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

14 of 15

Yes

Yes

12 of 15

No

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC1635.01

11 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190C4O )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00001858

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091508GK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY003090

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07612

Mayetiola destructor

Hessian fly

Mdes\Mdes011040

Aedes aegypti

Yellow fever mosquito

Anopheles darlingi

American malaria mosquito

Adar\ADAC010107

Anopheles gambiae

Malaria mosquito

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ000967

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W09O4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008955

Danaus plexippus

Monarch butterfly

Dple\DPOGS209741-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL011342

Apis florea

Little honeybee

Aflo\2160513140

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001b06

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003981

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM472560

Rhodnius prolixus

Kissing bug

Rpro\RPRC008679

Cimex lectularius

Bed bug

Clec\CLEC002216

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000248

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_00681

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BL9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005449

Ixodes scapularis

Black-legged tick

Isca\ISCW000781

Stegodyphus mimosarum

African social velvet spider

Smim\KFM69299.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM64129.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g04070

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g01190

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g03960

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_231133

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0F0O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Vdac2_1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002025

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

7 of 10

4 of 10

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

mitochondrial disorders, VDAC-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

model of retinal degeneration

CEA

(Huang et al., 2015)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Lee et al., 2018)

ameliorates Parkinson's disease

modeled by Miro^UAS.Tag:MYC

(Lee et al., 2018)

ameliorates neurodegenerative disease

modeled by par-1^UAS.cUa

(Lee et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

VDAC2; voltage dependent anion channel 2

14 of 15

VDAC3; voltage dependent anion channel 3

14 of 15

VDAC1; voltage dependent anion channel 1

13 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

porin - ATPsynB

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynCF6

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynF

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynO

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynβ

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynγ

experimental knowledge based

(Guruharsha et al., 2011)

porin - ATPsynδ

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG10425

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG11857

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG13887

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG15098

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG33253

experimental knowledge based

(Guruharsha et al., 2011)

porin - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti bait coimmunoprecipitation, western blot

(Lee et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, western blot

(Lee et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

porin - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

porin - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

porin - Vha68-2

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting

(Sen et al., 2015)

experimental knowledge based

(Guruharsha et al., 2011)

porin - l(2)37Cc

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

porin - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Park et al., 2010)

suppressible

(Park et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Zhao and Wang, 2020)

External Data

Subunit Structure (UniProtKB)

Interacts with hexokinases.

(UniProt, Q94920 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60572

DroID - A comprehensive database of gene and protein interactions.

FBgn0004363

MIST (protein-protein) - An integrated Molecular Interaction Database

porin

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-43

Cytogenetic map

Sequence location

2L:10,847,227..10,850,848 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32B1-32B1

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32B-32B

(Mesilaty-Gross, 1999.1.20, Vito, 1997.7.29)

32B1-32B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

32B3-32B4

(determined by in situ hybridisation)

(Caggese et al., 1999, Oliva et al., 1998)

32B-32B

(determined by in situ hybridisation)

(Ryerse et al., 1997)

79D-79D

(determined by in situ hybridisation)

(Messina et al., 1996)

32B1-32B2

32B1--3

(BDGP Project Members, 1994-1999)

In situ hybridisation reveals a strong signal at 79D and a weaker signal at 31E.

(Messina et al., 1996)

A cDNA clone also showed hybridization to 31E, but less strongly than at 79B. This may indicate the presence of two porin genes.

(De Pinto, 1995.10.16)

Experimentally Determined Recombination Data

Location

2-43

(FlyBase, 2016)

2-36.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

18676

w¹¹¹⁸; PBac{WH}porin^f03616

80864

y¹, v¹; P{TKO.GS04768}attP40

28403

y¹ w^*; P{EP}porin^G13650

43356

w^*; P{GSV1}porin^C242 SmydA-3^C242

10563

y¹ w^67c23; P{lacW}porin^k05123/CyO

29572

y¹ v¹; P{TRiP.JF03251}attP2/TM3, Sb¹

67873

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05687}attP40

102643

y¹ w^67c23 ; P{lacW}porin^k08405 / CyO

111174

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}porin^k05123 P{neoFRT}40A/CyO y⁺

v330138

P{VSH330138}attP40

v101336

P{KK107645}VIE-260B

v318633

PBac{fTRG00644.sfGFP-TVPTBF}VK00033

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (403)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_GH11331

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004363

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004363

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34500

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Zhao and Wang, 2020, Leggio et al., 2018, Park et al., 2010, Oliva et al., 2002)

monoclonal

(Banerjee and Chinthapalli, 2014)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: porin CG6647

(Li, 1999.11)

Source for database merge of

Source for merge of: porin l(2)k05123

(Spradling et al., 1999)

Source for merge of: porin anon-WO02059370.56

(FlyBase, 1996-)

Additional comments

Source for merge of porin anon-WO02059370.56 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

porin encodes a voltage-dependent and anion-selective channel.

(Aiello et al., 2004)

porin has been cloned and sequenced. It has been isolated from an expression library using an antibody against tobacco hornworm VDAC protein as a probe. Comparison of DNA sequence with that from FBrf0087605 reveals there is one porin gene, and two isolated cDNAs.

(Ryerse et al., 1997)

porin cDNA is isolated and characterised. Present results suggest porin may be a member of a multigene family.

(Messina et al., 1996)

porin has been characterised and compared to other mitochondrial porins.

(de Pinto et al., 1989)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 70 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34500

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94920

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PD75

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004363

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004363

DRscDB

34500/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0004363

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6647

Flygut - An atlas of the Drosophila adult midgut

FBgn0004363

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34500

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006408

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6647

MARRVEL_MODEL

34500

modMine - A data warehouse for the modENCODE project

FBgn0004363

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10563-porin[k05123]

BioGRID - A database of protein and genetic interactions.

60572

DroID - A comprehensive database of gene and protein interactions.

FBgn0004363

DRSC - Results frm RNAi screens

FBgn0004363

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6647-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004363

FlyMine - An integrated database for Drosophila genomics

FBgn0004363

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

porin

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG6647

(Graham et al., 2017, Kolkhof et al., 2017, Meadows, 2017.1.11, Çiçek et al., 2016, Jaspers et al., 2012, Tsubouchi et al., 2012, Cammarato et al., 2011, Müller et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Hughes et al., 2008, Cermelli et al., 2006, Guarino et al., 2006, Molnar et al., 2006, Terhzaz et al., 2006, Regulski et al., 2004)

DVDAC

(Komarov et al., 2004)

DmPorin1

(Aiello et al., 2004)

DmVDAC

(Blachly-Dyson et al., 1997, Ryerse et al., 1997)

POR-1

(Vito, 1997.7.29)

PORIN

(Sieber et al., 2016, Alonso et al., 2005)

Porin

(Chen et al., 2020, Ham et al., 2020, Lee et al., 2018, Martinez et al., 2017, Yoon et al., 2017, Lee et al., 2016, Gehrke et al., 2015, Sen et al., 2015, Peralta et al., 2012, Robinson et al., 2010)

VDAC

(Lee et al., 2016, Khalil et al., 2015, DeVorkin et al., 2014, Rival et al., 2011, Park et al., 2010, Komarov et al., 2004, Oliva et al., 1998, Ryerse, 1996.9.10)

VDAC1

(Vos and Klein, 2021, Sieber et al., 2016)

Vdac

Vdac1

(Graham et al., 2017)

anon-WO02059370.56

cg6647

(Park et al., 2010)

l(2)k05123

(Mesilaty-Gross et al., 1999, BDGP Project Members, 1994-1999)

porin

(Solana-Manrique et al., 2021, Hope et al., 2020, Kanellopoulos et al., 2020, Meltzer et al., 2019, Yuan et al., 2019, Zhou et al., 2019, Al-Ramahi et al., 2018, Liu et al., 2018, Valadas et al., 2018, Manning et al., 2017, Transgenic RNAi Project members, 2017-, M'Angale and Staveley, 2016, Sarov et al., 2016, Sen and Cox, 2016, Foriel et al., 2015, Huang et al., 2015, Liu and Secombe, 2015, Chen et al., 2014, Deivasigamani et al., 2014, Mukherjee et al., 2014, de Celis et al., 2013.9.11, Kwon et al., 2013, Thomae et al., 2013, Zhang et al., 2013, Duboff et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Peralta et al., 2012, Tsubouchi et al., 2012, Friedman et al., 2011, Graham et al., 2010, Groebe et al., 2010, Müller et al., 2010, Park et al., 2010, Wasbrough et al., 2010, Tan et al., 2009, Kaltenbach et al., 2007, Lee et al., 2007, Beller et al., 2006, Christensen and Cook, 2006.12.5, Molnar et al., 2006, Terhzaz et al., 2006, Graham and Craigen, 2005)

Name Synonyms

Porin

(Vásquez-Procopio et al., 2020, Khalil et al., 2015, Zhang et al., 2013, Pedersen et al., 2010, Robinson et al., 2010)

Porin 1

(Guarino et al., 2006)

VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL

(Hull et al., 2015)

Voltage-dependent anion-selective channel

mitochondrial porin

(Komarov et al., 2004)

porin

(Bahhir et al., 2019, Jaspers et al., 2012, Tsubouchi et al., 2012, Cammarato et al., 2011, Sun et al., 2011, Graham et al., 2010, Craigen and Graham, 2008, Kaltenbach et al., 2007, Guarino et al., 2006)

porin 1

(Specchia et al., 2008)

voltage-dependent anion channel

(Khalil et al., 2015)

Secondary FlyBase IDs

FBgn0016759
FBgn0022189
FBgn0024919
FBgn0066003

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (147)

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