FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
cuff, Boot
FlyBase ID
FBig0000124004
Interaction Network
Interactions Browser links
cuff network
Boot network
Reported Interactions
FBrf0263998-8.CL.FM
Reference
Ismail et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Boot 

Bootlegger

bait

PH-mCherry tag

cuff 

Cutoff

prey

mEGFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Ismail et al., 2025)

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

(Ismail et al., 2025)
FBrf0263998-9.CL.FM
Reference
Ismail et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
cuff 

Cutoff

bait

PH-mCherry tag

Boot 

Bootlegger

prey

mEGFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Ismail et al., 2025)

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

(Ismail et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)