FB2026_03 , released September 17, 2026
Result: BCM_1_E2-16hr_junctions
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General Information
Name
BCM_1_E2-16hr_junctions
Species
D. melanogaster
Result type
exon junction identification
FlyBase ID
FBlc0000075
Project
Data Provider
Title
Exon junction identification from RNA-Seq assays of D. melanogaster, Canton-S strain, embryo (2-16 hr).
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Canton-S
    (Daines et al., 2011)
    Stage
    blastoderm stage
    (Daines et al., 2011)
    gastrula stage
    (Daines et al., 2011)
    early extended germ band stage
    (Daines et al., 2011)
    late extended germ band stage
    (Daines et al., 2011)
    dorsal closure stage
    (Daines et al., 2011)
    late embryonic stage
    (Daines et al., 2011)
    Sex
    Tissue isolated
    organism
    (Daines et al., 2011)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    BS_BCM_E2-16hr
    whole organism
    D. melanogaster, Canton-S strain, embryo (2-16 hr), source for RNA.
    Data Analyzed
    Assay
    RNA-Seq
    (Daines et al., 2010.2.8)
    Assay methods
    random priming
    (Daines et al., 2010.2.8)
    Illumina sequencing
    (Daines et al., 2010.2.8)
    poly(A) RNA isolation
    (Daines et al., 2010.2.8)
    paired-end layout
    (Daines et al., 2010.2.8)
    Key genes
    Protocol

    Whole body samples were homogenized in 1ml of Trizol per 50-100 mg of tissue to isolate total RNA. Polyadenylated mRNA was then isolated from 20ug total RNA using an mRNA Purification Kit (Invitrogen). Double-stranded cDNA was made using Superscript Double-Stranded cDNA Synthesis Kit (Invitrogen) and random hexamer primers. Size fractionated 200-400bp cDNAs were used for Solexa library construction (paired end layout).

    Mode of Assay

    Read length (bases):75

    The cDNA library was characterized by high-throughput sequencing (Illumina GAII).

    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    RNA-Seq_GSE24324_E4-16
    RNA-Seq
    RNA-Seq of D. melanogaster, Canton-S strain, embryo (2-16 hr).
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis
    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    BCM_1_E2-16hr_junctions
    Embryo (E2-16hr)
    (Daines et al., 2011, Daines et al., 2010.2.8)
    embryo.2_E2-16hr
    (Daines et al., 2010.2.8)
    Name Synonyms
    Exon junction identification from RNA-Seq assays of D. melanogaster, Canton-S strain, embryo (2-16 hr).
    (Daines et al., 2010.2.8)
    Secondary FlyBase IDs
      References (2)