FB2026_03 , released September 17, 2026
Assay: RNA-Seq_GSE24324_E4-16
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General Information
Name
RNA-Seq_GSE24324_E4-16
Species
D. melanogaster
Assay type
RNA-Seq
FlyBase ID
FBlc0000963
Project
Data Provider
Title
RNA-Seq of D. melanogaster, Canton-S strain, embryo (2-16 hr).
Accessions
Biosample Source
Overview
Strain
Canton-S
(Daines et al., 2011)
Stage
blastoderm stage
(Daines et al., 2011)
gastrula stage
(Daines et al., 2011)
early extended germ band stage
(Daines et al., 2011)
late extended germ band stage
(Daines et al., 2011)
dorsal closure stage
(Daines et al., 2011)
late embryonic stage
(Daines et al., 2011)
Sex
Tissue isolated
organism
(Daines et al., 2011)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation
Biosamples used (1)
Biosample
Type
Title
BS_BCM_E2-16hr
whole organism
D. melanogaster, Canton-S strain, embryo (2-16 hr), source for RNA.
Assay Details
Methods
poly(A) RNA isolation
(Daines et al., 2010.2.8)
random priming
(Daines et al., 2010.2.8)
paired-end layout
(Daines et al., 2010.2.8)
Illumina sequencing
(Daines et al., 2010.2.8)
Key genes
Protocol

Whole body samples were homogenized in 1ml of Trizol per 50-100 mg of tissue to isolate total RNA. Polyadenylated mRNA was then isolated from 20ug total RNA using an mRNA Purification Kit (Invitrogen). Double-stranded cDNA was made using Superscript Double-Stranded cDNA Synthesis Kit (Invitrogen) and random hexamer primers. Size fractionated 200-400bp cDNAs were used for Solexa library construction (paired end layout).

Mode of Assay

Read length (bases):75

(Daines et al., 2010.2.8)

The cDNA library was characterized by high-throughput sequencing (Illumina GAII).

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RNA-Seq_GSE24324_E4-16
Name Synonyms
RNA-Seq of D. melanogaster, Canton-S strain, embryo (2-16 hr).
(Daines et al., 2010.2.8)
Secondary FlyBase IDs
    References (2)