Reagent: TI_set

General Information

Name

TI_set_P{EP}.G

Species

D. melanogaster

Reagent type

transgenic insertion collection

FlyBase ID

FBlc0000525

Project

Gene_Disruption_Project

Created by

GDP Transposons

Vector

Title

A set of P-element construct P{EP} insertions.

Accessions

Overview

Description

A set of transgenic insertion stocks derived by TE mobilization using the using the P-element construct P{EP}. The P{EP} construct construct carries a w^+mC mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue (FBrf0090768). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

(Bellen et al., 2011)

Available from

Bloomington Drosophila Stock Center

Biosample Source

Overview

Strain

Stage

Sex

Tissue isolated

Other tissues studied

Cell component

Cell line

Key genes

Methods

Sample preparation

Reagent Details

Methods

Transgenic Construct used

P{EP}

Protocol

Markers in stocks (as originally submitted to the BDSC):w^*; w¹¹¹⁸ ; y¹ w^*.

(Matthews, 2013.1.25)

Mode of Assay

Data analysis

1685 strains produced in a large-scale screen by GenExel (now Aprogen) were evaluated; flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 1136 selected lines were balanced and added to the Gene Disruption Project collection.

(Bellen et al., 2011)

Comments

The Scer\GAL4 driver in the P{EP} construct cannot be activated in the female germ line (FBrf0128569).

(Bellen et al., 2011)

Associated Data

Size

Associated features

1,337 Insertion(s)

Download

Files

Additional Information

GDP Transposons