FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Result: NIH_mRNA_Dpse_M_testis
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General Information
Name
NIH_mRNA_Dpse_M_testis
Species
D. pseudoobscura pseudoobscura
Result type
RNA-seq profile
FlyBase ID
FBlc0000602
Project
Data Provider
Title
RNA-Seq coverage profile for D. pseudoobscura, adult male testis.
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    adult stage
    (GEO curators and FlyBase curators, 2013)
    Sex
    male
    (GEO curators and FlyBase curators, 2013)
    Tissue isolated
    testis
    (GEO curators and FlyBase curators, 2013)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    BS_NIH_Dpse_AM_testis
    tissue
    D. pseudoobscura, adult male testis, source for RNA.
    Data Analyzed
    Assay
    RNA-Seq
    (Chen et al., 2013.5.31)
    Assay methods
    paired-end layout
    (Chen et al., 2013.5.31)
    Illumina sequencing
    (Chen et al., 2013.5.31)
    random priming
    (Chen et al., 2013.5.31)
    poly(A) RNA isolation
    (Chen et al., 2013.5.31)
    Key genes
    Protocol
    Mode of Assay

    Read length (bases):75

    (Chen et al., 2013.5.31)
    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    RNA-Seq_mE4050_Dpse_AM_testis
    RNA-Seq
    RNA-Seq of D. pseudoobscura, adult male testis.
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    unstranded profile
    (Chen et al., 2013.5.31)
    uniquely mapping read alignment
    (Chen et al., 2013.5.31)
    Reference Genome
    Reference Annotation
    Data analysis

    Only Illumina GAII data was used. Raw reads were trimmed to 75 nt before mapping (shorter reads were removed) and mapped using TopHat (v2.0.8b) with parameters: -g 1 -r 150 --solexa1.3-quals (except for data from head samples where parameters were -g 1 -r 150).

    Comments
    Associated Data
    Size
    350836139 Uniquely aligned reads; as reported.
    (Chen et al., 2013.5.31)
    Files
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    Dpse_0121.94_M_Testis
    (Chen et al., 2013.5.31)
    Dpse_0121.94_Testes_and_Reproductive_Tract
    (GEO curators and FlyBase curators, 2013)
    NIH_mRNA_Dpse_M_testis
    Name Synonyms
    RNA-Seq coverage profile for D. pseudoobscura, adult male testis.
    (Chen et al., 2013.5.31)
    Secondary FlyBase IDs
      References (2)