Data from many short RNA-Seq assays from multiple, independent studies have been analyzed in parallel and consolidated into data for 26 cell lines. miRNA expression was computed by first identifying all perfectly-mapped reads that reside entirely within the coordinates flanked by the miRNA sequences + 2nt flanking region; coordinates for the mature and star sequences are provided in "dmeMirnaInfoMature_dm6.bed" and "dmeMirnaInfoStar_dm6.bed". For reads mapping to multiple locations, the coverage values were equally weighted across all mapping sites. The mapping results are provided in two files, "dmeMatureCoverage_v3.txt" and "dmeStarCoverage_v3.txt", that contain the number of mapped reads per library for the mature sequence and the star sequence, respectively. The "summarizeCoverage.R" R script combines data from all libraries for a given condition to calculate the expression for each miRNA in terms of mapped miRNA reads per million miRNA reads (RPMM).