FB2026_03 , released September 17, 2026
Assay: ChIP-Seq_Furlong_Mef2_E11-12
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General Information
Name
ChIP-Seq_Furlong_Mef2_E11-12
Species
D. melanogaster
Assay type
ChIP-Seq
FlyBase ID
FBlc0000998
Project
Data Provider
Title
Chip-Seq of Mef2 from D. melanogaster, embryo (6-8 hr AEL).
Accessions
Biosample Source
Overview
Strain
Stage
early extended germ band stage
(Bonn et al., 2012)
late extended germ band stage
(Bonn et al., 2012)
Sex
Tissue isolated
organism
(Bonn et al., 2012)
Other tissues studied
Cell component
Cell line
Key genes
Methods
targeted cell labeling
(Bonn et al., 2012)
Sample preparation

A strain expressing mesodermally expressed SBP-tagged His2B was used but the entire embryo was used for subsequent ChIP (no FACS-enrichment of mesodermal nuclei).

Biosamples used (1)
Biosample
Type
Title
BS_Furlong_E6-8h_organism
whole organism
D. melanogaster, embryo (6-8 hr AEL), source for chromatin.
Assay Details
Methods
single-end layout
(Bonn et al., 2012)
Illumina sequencing
(Bonn et al., 2012)
Key genes
(bait_protein)
Protocol

Chromatin immunoprecipitation of Mef2.

(Bonn et al., 2012)

Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

Mode of Assay

Chromatin immunoprecipitated DNA was analyzed by next generation sequencing.

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
ChIP-Seq_Furlong_Mef2_E11-12
Name Synonyms
Chip-Seq of Mef2 from D. melanogaster, embryo (6-8 hr AEL).
(Bonn et al., 2012)
Secondary FlyBase IDs
    References (2)