FB2026_03 , released September 17, 2026
Assay: mock_RIP_E1-6_stau-GFP
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General Information
Name
mock_RIP_E1-6_stau-GFP
Species
D. melanogaster
Assay type
RIP-Seq
FlyBase ID
FBlc0001695
Project
Data Provider
Title
Mock immunoprecipitation for stau-GFP RNA immunoprecipitation.
Accessions
Biosample Source
Overview
Strain
Stage
pre-blastoderm stage
(Laver et al., 2013)
blastoderm stage
(Laver et al., 2013)
gastrula stage
(Laver et al., 2013)
Sex
Tissue isolated
organism
(Laver et al., 2013)
Other tissues studied
Cell component
Cell line
Key genes
(transgene_used)
Methods
Sample preparation

Flies were maintained at 25oC and a 0-3 hour embryo collection was collected.

Biosamples used (1)
Biosample
Type
Title
BS_E1-6_stau-GFP_RIP
whole organism
D. melanogaster, stau-GFP line in stau mutant background, embryo (0-3 hr), source of extract for stau-GFP RIP.
Assay Details
Methods
Key genes
Protocol

The mock immunoprecipitation used an anti-FLAG antibody. RNA in coimmunoprecipitation was purified using Trizol reagent. Double-stranded cDNA was prepared from isolated RNA using oligo-dT primers and labelled for subsequent hybridization.

(Laver et al., 2013)
Mode of Assay

Labelled cDNA libraries were characterized by hybridization to custom-designed NimbleGen array (GPL10539).

(Laver et al., 2013)
Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
mock_RIP_E1-6_stau-GFP
Name Synonyms
Mock immunoprecipitation for stau-GFP RNA immunoprecipitation.
(Laver et al., 2013)
Secondary FlyBase IDs
    References (2)