Open reading frames were transferred from the BDGP Drosophila melanogaster expression-ready clone set (FBrf0213191) to the pMK33-C-FLAG-HA acceptor vector (see DPiM_cDNA_bait collection report). Each clone was transiently transfected into a 54 ml culture of Drosophila S2R+ cells. Protein expression was induced with 0.35 mM CuSO4 for 24 hours and nuclear extracts were prepared (PMID: 6828386) then diluted 1:1 with dialysis buffer (20mM HEPES [pH 7.6], 20% glycerol, 100 mM KCl, 2mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 0.25 mM PMSF and Roche Complete protease inhibitor).