FB2026_03 , released September 17, 2026
Project: scRNAseq_2017_Li
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General Information
Name
scRNAseq_2017_Li
Species
D. melanogaster
Project type
transcriptome
FlyBase ID
FBlc0003791
Parent Project
    Data Provider
    [Luo Lab, Howard Hughes Medical Institute](https://web.stanford.edu/group/luolab/)
    Title
    Single-cell RNA-seq study of pupal olfactory projection neurons
    Accessions
    Overview
    Description

    A single-cell transcriptomic study of the diverse populations of olfactory projection neurons in the Drosophila pupal brain.

    (Li et al., 2017)
    Project attributes
    tissue type study
    (Li et al., 2017)
    Biosample type
    isolated cells
    (Li et al., 2017)
    Assay type
    single-cell RNA-Seq
    (Li et al., 2017)
    Reagent type
    Result type
    cell clustering analysis
    (Li et al., 2017)
    Key genes
    GO term(s)
    brain development
    (Li et al., 2017)
    SO term(s)
    Details
    Sample preparation
    Protocol
    Mode of Assay
    Data analysis

    Reads were aligned to the Drosophila melanogaster reference genome using STAR v2.4.2a (Dobin et al., 2013). Uniquely mapped reads that overlap with genes were counted using HTSeq-count v0.7.1 (Anders et al., 2015), with cells having fewer than 300,000 uniquely mapped reads removed. Principal component analysis was performed using the top 500 most variable genes; typically 7 to 12 principal components were used for further analysis.

    (Li et al., 2017)
    Comments
    Files
    Additional Information

    The EMBL-EBI's Single Cell Expression Atlas provides cell-level annotations, clustering data, raw and normalised read counts, and putative marker genes.

    (Li et al., 2017)
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    scRNAseq_2017_Li
    Name Synonyms
    Single-cell RNA-seq study of pupal olfactory projection neurons
    (Li et al., 2017)
    Secondary FlyBase IDs
      References (1)