FB2026_03 , released September 17, 2026
Result: scRNAseq_2017_Li_GH146_seq_clustering
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General Information
Name
scRNAseq_2017_Li_GH146_seq_clustering
Species
D. melanogaster
Result type
cell clustering analysis
FlyBase ID
FBlc0003794
Project
Data Provider
Title
Clustering analysis of GH146-labeled olfactory projection neurons from pupal brain
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    pupal stage
    (Li et al., 2017)
    Sex
    Tissue isolated
    pupal brain
    (Li et al., 2017)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    multi-individual sample
    (Li et al., 2017)
    cell isolation
    (Li et al., 2017)
    Sample preparation
    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    scRNAseq_2017_Li_GH146
    isolated cells
    GH146-labeled olfactory projection neurons from pupal brain
    Data Analyzed
    Assay
    single-cell RNA-Seq
    (Li et al., 2017)
    Assay methods
    Smart-seq2
    (Li et al., 2017)
    Illumina sequencing
    (Li et al., 2017)
    Key genes
    Protocol
    Mode of Assay
    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    scRNAseq_2017_Li_GH146_seq
    single-cell RNA-Seq
    Single-cell RNA-seq of GH146-labeled olfactory projection neurons from pupal brain
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    EMBL-EBI SCEA standard analysis method
    (Li et al., 2017)
    Reference Genome
    Reference Annotation

    Dmel R6.28

    (Li et al., 2017)
    Data analysis

    Reads were aligned to the Drosophila melanogaster reference genome using STAR v2.4.2a (Dobin et al., 2013). Uniquely mapped reads that overlap with genes were counted using HTSeq-count v0.7.1 (Anders et al., 2015), with cells having fewer than 300,000 uniquely mapped reads removed. Principal component analysis was performed using the top 500 most variable genes; typically 7 to 12 principal components were used for further analysis.

    Comments
    Associated Data
    Size
    1164 cells
    (Li et al., 2017)
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    scRNAseq_2017_Li_GH146_seq_clustering
    Name Synonyms
    Clustering analysis of GH146-labeled olfactory projection neurons from pupal brain
    (Li et al., 2017)
    Secondary FlyBase IDs
      References (1)