P{1151}GAL4; +; P{VALIUM20-mCherry}attP2
Wandering third instar larvae expressing Disc\RFPdsRNA.mCherry.UAS.VALIUM20 under the control of Scer\GAL41151 were harvested between 110 and 135 h after egg-laying. The notum and hinge regions of wing discs were dissected and cells were dissociated with collagenase and trypsin in Rinaldini solution.
