A characterization of the diverse populations of cells in the adult Drosophila midgut.
Reads were processed using Cell Ranger v3.0.2 (10x Genomics). They were mapped to the Drosophila melanogaster reference genome BDGP6. For quality filtering, cellls with total UMI below 200 or above 2,000, cells with less than 500 genes expressed, and cells with more than 8% of mitochondrial genes detected, were excluded. Clustering analysis and automatic cell annotation were performed with the Monocle 3 v0.2.1 and Garnett v0.1.17 R packages, respectively.
The EMBL-EBI's Single Cell Expression Atlas provides cell-level annotations, clustering data, raw and normalised read counts, and putative marker genes.
