The thoraces from thirty adult males were dissected and homogenized. Cells were mechanically dissociated and about ten thousand nuclei per genotype were collected.
Dmel R6.32
Reads were processed using Cell Ranger v7.0.0 (10x Genomics). Further analysis was performed with Seurat v4.3.0. For quality filtering, cells less than 100 UMIs or less than 100 genes were removed. After UMAP dimensionality reduction, clustering analysis was performed with a resolution of 0.3 and clusters were annotated by comparing the top markers with data from the Fly Cell Atlas dataset.
