SalI/XhoI (inclusive)
BglII/BamHI (inclusive)
report comment: BamHI cloning site flanked by NotI siteon left and SfiI site on right.
construct_comment: Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in medium containing 5% sucrose.BamHI cloning site flanked by T7 promoter and NotI site on left; flanked by SP6 promoter and SfiI site on right.
report comment: BamHI cloning site flanked by NotI siteon left and SfiI site on right.
construct_comment: Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in medium containing 5% sucrose.BamHI cloning site flanked by T7 promoter and NotI site on left; flanked by SP6 promoter and SfiI site on right.