construct_comment: DT-AtsM is a cold-sensitive mutation of the diphtheria toxin A subunit (DT-A; toxin gene carried by the corynebacteriophage of C. diphtheriae).Selectivity imposed both by temperature sensitivity and by substitution of different promoter sequences.
construct_comment: One of a series of DT-A cell ablation vectors containing the ninaE promoter.The ninaE promoter can be excised and replaced using the flanking EcoRI and KpnI sites.
sequence comment: CAUTION: Extent of SV40 sequences may be incorrect.
mapping_comment: CAUTION: Extent of SV40 sequences may be incorrect.
construct_comment: DT-AtsM is a cold-sensitive mutation of the diphtheria toxin A subunit (DT-A; toxin gene carried by the corynebacteriophage of C. diphtheriae).Selectivity imposed both by temperature sensitivity and by substitution of different promoter sequences.
construct_comment: One of a series of DT-A cell ablation vectors containing the ninaE promoter.The ninaE promoter can be excised and replaced using the flanking EcoRI and KpnI sites.