FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: pP{GreenH-Pelican}
Open Close
General Information
Symbol
pP{GreenH-Pelican}
FlyBase ID
FBmc0002844
Feature type
engineered_plasmid
Component Allele(s)
Size
10.189
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Cloning vector used to generate transgenic constructs for promoter/enhancer analysis. Contains a reporter cassette that is composed of a multiple cloning site upstream of a minimal Hsp70 promoter followed by sequence encoding the EGFP fluorescent protein. The cassette is flanked by a pair of gypsy insulators to minimise position effects.

(Barolo et al., 2000)
Construct components
Component
Use(s)
Encoded product / tool
EGFP
Marker(s)
w+mC
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
AF242363
(bases )
Segments and Size
Total Size

10.189

(Barolo, 2000.3.8)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
pP{YellowH-Pelican}
(Liang, 2003.9.16)
Comments

construct_comment: Cloning sites based on output of NEBcutter V2.0.

(Barolo, 2000.3.8)

report comment: Designed to avoid inappropriate activation of reporter by white regulatory sequences.gypsy\su(Hw)BR430 insulator sequences flank reporter cassette.

(Barolo et al., 2000)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pGHP
(Barolo et al., 2004)
pP{GreenH-Pelican}
(Barolo, 2000.3.8, Barolo et al., 2000, FlyBase, 1996-)
Secondary FlyBase IDs
    References (6)