construct_comment: piggyBac vector for cloning targeted gene behind UASG sites.Comparable to pP{UAST}, with identical 5xUAS segment, Hsp70 TATA box and transcriptional start; smaller SV40 polyA segment following polylinker.Mini-white selectable marker is similar to, but not identical to, w+mC and w+mPB.Cloning sites based on output of NEBcutter V2.0.
construct_comment: piggyBac vector for cloning targeted gene behind UASG sites.Comparable to pP{UAST}, with identical 5xUAS segment, Hsp70 TATA box and transcriptional start; smaller SV40 polyA segment following polylinker.Mini-white selectable marker is similar to, but not identical to, w+mC and w+mPB.Cloning sites based on output of NEBcutter V2.0.