FB2026_02 , released June 18, 2026
Recombinant Construct: pSacBII
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General Information
Symbol
pSacBII
FlyBase ID
FBmc0002975
Feature type
engineered_plasmid
Component Allele(s)
Size
16.011
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
U09128
(bases 1-16011 )
Segments and Size
Total Size

16.011

(Hartl et al., 1994)
Left end

BamHI (inclusive)

(Hartl et al., 1994)
Right end

BamHI

(Hartl et al., 1994)
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
pBACe3.6
(FlyBase, 1992-)
Comments

construct_comment: Portion of P1 cloning vector P1(pAd10-sacBII) packaged after insertion of cloned DNA. Equivalent to P1(pAd10-sacBII) without the Ad10 stuffer fragment (after loxP recombination).Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in high concentrations of sucrose.Reference: Pierce et al., 1992, Proc. Natl. Acad. Sci. USA 89:2056-2060 .

(Hartl et al., 1994)

construct_comment: Equivalent to P1(pAd10-sacBII) without the Ad10 stuffer fragment (after loxP recombination).

(Hartl et al., 1994)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pAd
(BDGP Project Members, 1994-1999)
pSacBII
(FlyBase, 1996-, Roe, 1994.4.20)
Secondary FlyBase IDs
  • FBtp0001097
References (5)