Small shuttle vector with large number of cloning sites; MCS flanked by 8-base cutter sites. The SphI site has been eliminated from the MCS, since it contains an ATG. Use of shuttle vector with different antibiotic resistance eliminates need to gel-purify fragment of interest.
Small shuttle vector with large number of cloning sites; MCS flanked by 8-base cutter sites. The SphI site has been eliminated from the MCS, since it contains an ATG. Use of shuttle vector with different antibiotic resistance eliminates need to gel-purify fragment of interest.