One of a series of cloning vectors designed to allow recombination-mediated genomic insertion via the phiC31 system; the paired Gateway recombination cassettes within the vector are flanked by gypsy\su(Hw)BR insulator sequences, to minimize variation in expression due to genomic site-specific effects. This vector includes 10 Scer\UAS GAL4-binding sites followed by an Hsp70 promoter, and has two Gateway cassettes oriented in opposite directions to allow insertion of inverted fragments.
One of a series of cloning vectors designed to allow recombination-mediated genomic insertion via the phiC31 system; the paired Gateway recombination cassettes within the vector are flanked by gypsy\su(Hw)BR insulator sequences, to minimize variation in expression due to genomic site-specific effects. This vector includes 10 Scer\UAS GAL4-binding sites followed by an Hsp70 promoter, and has two Gateway cassettes oriented in opposite directions to allow insertion of inverted fragments.