FB2026_02 , released June 18, 2026
Recombinant Construct: pCR8/GW/TOPO
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General Information
Symbol
pCR8/GW/TOPO
FlyBase ID
FBmc0003110
Feature type
engineered_plasmid
Component Allele(s)
Size
2.817
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

2.817

(FlyBase, 1992-)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Allows initial cloning of a Taq-generated PCR product via TOPO ligation; insertion site is flanked by attL1 and attL2 sites to allow use of Gateway cloning systems. Also includes primer binding sites to allow efficient sequencing of the insert.

(FlyBase, 1992-)

Commercially available from Invitrogen.

(FlyBase, 1992-)

Additional information about the TOPO system for using topoisomerase for cloning: http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cloning/topo/topo-resources/the-technology-behind-topo-cloning.html

(FlyBase, 1992-)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pCR8/GW/TOPO
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)