pWALIUM22 allows cloning of short hairpins as annealed synthetic oligos, targeted against specific genes via the production of short hairpin microRNAs; contains white as a selectable marker; the P-transposase core promoter; a multiple cloning site (MCS) for cloning shRNAs in a mir-1 scaffold; a ftz 3'UTR intron followed by a fs(1)K10 3'UTR to facilitate shRNA processing and export from the nucleus; two pentamers of UAS, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.
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'WALIUM' stands for White-AttB-Loxp-Intron-UAS-MCS.
See TRiP(http://fgr.hms.harvard.edu/trip-plasmid-vector-sets) for downloadable maps, sequences and cloning protocols. pWALIUM22 triggers effective RNAi knockdown and corresponding phenotypes in the female germline; it is less effective in somatic cells.