Gateway destination vector. Contains a synthetic core promoter followed by sequence encoding nuclease-dead Spyo\Cas9 ('dCas9') that contains a single nuclease-attenuating mutation in each of the RuvC and HNH domains. The coding sequence is tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame. A tissue-specific enhancer/promoter region can be introduced into this vector to generate a tissue-specific dCas9 expression construct which can then be inserted into the genome using phiC31:int integrase. The transgene can then be used for transcription interference (CRISPRi) in combination with an sgRNA that targets dCas9 to a promoter; the promoter-bound dCas9 can sterically interfere with the formation of the pre-initiation complex, disrupting transcription.