FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: pCFD5-NS
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General Information
Symbol
pCFD5-NS
FlyBase ID
FBmc0004069
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

The pCFD5-NS dual-expression plasmid is designed for expression of a prime editing guide RNA (pegRNA) plus a nicking sgRNA for use in the 'Prime editor 3' (PE3) system. The pegRNA and sgRNA are expressed under the control of the strong, ubiquitous U6:3 (snRNA:U6:96Ac) promoter as a single polycistronic tRNA-based cassette, and the separate pegRNA and sgRNA components are then released by the endogenous tRNA-processing machinery. The vector contains an attB site, allowing integration into an attP-containing docking site in the genome to produce a transgenic construct.

(Bosch et al., 2021)
Construct components
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Also carries
attB
Marker(s)
v+t1.8
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pCFD5-NS
(Bosch et al., 2021, FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)