Abstract
A large transposable element (TE) comprising the white-apricot and roughest genes has been found to transpose to well over a hundred sites scattered over the Drosophila genome. We report the cloning of the essential parts of several TEs. TE98 and TE28 sequences were cloned by ;walking' along the chromosome from the previously cloned heatshock genes. The ends of the TEs are characterized by dispersed repetitive elements belonging to the foldback (FB) family. FB elements are also associated with two independently isolated transposable elements originating from the white locus, Tp w-1 and Tp w. The strong correlation between FB elements and large composite transposons suggests that a pair of these elements can mobilize large intermediary DNA segments. One particular FB family member, FB-NOF, is associated with TE28, the white-crimson (w) mutant, the w-derived Tp w-1 and probably also with Tp w. A unique sequence located close to the white end of TE28 was used to clone the borders of TE77 and the surrounding sequences in the bithorax region, indicating that the TE can be used as a probe for gene isolation. Some evolutionary implications of the large composite transposons are discussed.
