Engels, W.R., Preston, C.R., Johnson-Schlitz, D.M. (1994). Long-range cis preference in DNA homology search over the length of a Drosophila chromosome. Science 263(5153): 1623--1625.
FlyBase ID
FBrf0068483
Publication Type
Research paper
Abstract
P element-induced chromosome breakage on the X chromosome of Drosophila melanogaster was repaired six times more frequently when a homologous template was located anywhere on the X chromosome rather than on an autosome. Cis-trans comparisons confirmed that recombinational repair was more frequent when the interacting sequences were physically connected. These results suggest that the search for homology between the broken ends and a matching template sequence occurs preferentially in the cis configuration. This cis advantage operates over more than 15 megabases of DNA.
