Subject: Re: Inversion name, carries
        Reply to:   RE>Inversion name, carries Hobo
See info below.  Write again if you have questions.  Ron
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Dear Ronald,
  I am a member of FlyBase at the Cambridge, England site and I am
currently working on the curation of constructs and vectors.  Before
curating any recent journals I am having to return to the original
papers where the most detail about vector construction is given.  In
your paper about the identification of a fully functional hobo
transposable element (Embo J. 8: 211--217) you discuss an inversion
between dpp and an unknown locus in region 22E1--22E2.  Unfortunately
you do not name this.
  While searching my aberrations file I found:
ab symbol : In(2L)dpps5
ab name   : Inversion (2L) decapentaplegic
reference : Gelbart in Lindsley and Zimm, 1992
reference : Gelbart
cytology  : 21E1-21E2;22F1-22F2
mutagen   : X-ray
I realise the first breakpoint is wrong but it is the nearest inversion
description I could find to the one you used.  Is this the one?
If not could you please give me a complete description so I can
incorporate it into FlyBase.  I need the inversion to give a progenitor
chromosome for the BamHI HindIII fragment that carried the Hobo element.
The information I require:
Inversion name:  In(2L)dpp^d1 (that's superscripted d1--the very first dpp
allele Bill gelbart recovered--It's in L & Z.)
Inversion cytology:  see L & Z
Has the unknown locus at 22E1--22E2 been named?  Not to my knowledge
Which breakpoint is within the BamHI HindIII fragment?  Breakpoint is at
105.0 on the dpp molecular map.  Har1 contains the 105 to 106.3 sequences of
dpp (that is, the sequences centromere proximal of the breakpoint).  I hope
this answers your question.
21     ? dpp      ? dpp      60
--------+----------+-----------
        ^    or    ^
        |          |
Is the Hobo insertion absolutely at the inversion breakpoint?  Yes
Thank you very much,
Eleanor Whitfield.