Subject: query about some emc aberrations
Dear Dr. Ellis,
We have found some anomalies in our records for some aberrations you
isolated some years ago, and wonder if you could help.
(1) The Bloomington stock centre has three deficiencies for emc, which
they call Df(3L)emc5, emc18 and emc24.  Do you know where these names
come from?  We know that emc5 is the same as Df(3L)Ac14-8 cited in the
1993 Genetics paper by Castrillon et al., and we understand that 14-8
was your original isolation number.  Have there been any other mentions
of any of these three in the literature, to your knowledge?
(2) emc19, aka 29-2, is noted in the Red Book (and thence in FlyBase)
as associated with a T(2;3).  The breakpoints, unfortunately, are given
as 5B5-9;61C4.  Is it in fact a T(1;3) or is there a digit missing from
the cytology?
Many thanks in advance,
Aubrey de Grey
FlyBase
Organization: Emory University Biology Dept.
Subject:  Re: query about some emc aberrations
Priority: normal
1)  Sorry about the confusion with the deficiencies.  I hadn't noticed
how they were named.  It seems that Kathy named the deficiencies I sent
her by my stock list #s (H5, H18, H24).  This obviously won't  do.  The
stock that Bloomington calls Df(3L)emc5 is indeed the same as what the
Castrillon paper calls Df(3L)Ac14-8.  14-8 is my isolation # but I'm
not sure where they came up with Ac.  Apparently they got the stock
from Jim Posakony.  Each of these deficiencies were isolated as
revertants of emcD, aka Ach (sorry my email program doesn't do
superscripts).  My nomenclature (never published) was Df(3L)Ar14-8 (for
B's Df(3L)emc5), Df(3L)Ar12-1 (for B's Df(3L)emc18) and Df(3L)Ar11 (for
Df(3L)emc24 ).  I think this would be a reasonable nomenclature since
people who received the stocks from me will have my nomenclature.  I
don't know of any other publications that used these deficiencies.
However,  I have sent them to a number of labs.
2)  emc19 is  T(1;3)5B5-9; 61C4
3)  If it hasn't been changed yet, the cytological location for emc
should be 61D1-2 (by in situ localization of an emc cDNA probe)
Regards,
Hilary Ellis  
<original letter of Feb 11th 1993 to Bloomington>
Dear Kathy,
Here are the deficiency stocks.  The numbers on the vials are my lab
stock number.  The cytology and genotypes are below.  I have not checked
the cytology in several years, although other labs have used them during
that period.  Note that they all should have an emc phenotype.  Let me 
know if you have any problems with them.
Sincerely,
Hilary Ellis
H5 - Df(3L)61C3/4,62A8, red/TM2, emc2 pp Ubx es
H18 - Df(3L)61C,61F, red/TM2, emc2 pp Ubx es
H24 - Df(3L)61C3/4,61E, red/TM2, emc2 pp Ubx es'