Dear colleagues at Flybase, Upon your request (and I always wanted to do something about this any way), I checked flybase entries related to PTPase genes. Followings are short summary of my finding and recommendation. FlyBase entry 'Dlar' is mostly correct, except that the cytological location of the gene should be 38A1. It might be useful to add that the screw gene (scw) is located within the Dlar gene (within one of the Dlar intron), which is mentioned in reference FBrf0076510 by Arora, Levine, and O'Conner. FlyBase entry 'Ptp61F' has many problems. I understand that you do not like the prefix D for Drosophila genes, so calling this gene Ptp61F must be OK. In Function(s) of product, it should be 'cytosolic and nuclear protein tyrosine phosphatase', and delete the current description. In Synonym(s), you should only keep dPTP61F (i.e., delete DPTP, Pptp, and Ptp). In DNA/RNA AC no(s), M27699 must be removed. In Protein AC no(s), both PIR/B36182 and SWP/P16620 should be removed. In Reference, the first reference (FBrfOO50872) should be entirely removed. FBrf0058795 is correct. In gene Reference(s), FBrf0050872 should be removed. Then there will be several other DPTP entries (DPTP4E, 10D, 64D <up>= original DPTP</up>, and 99A) that must be added. For 10D and 99A, please contact Kai Zinn at Caltech .. For 4E, contact William Chia at National University of Singapore .. I will supply the information about 64D. Is there any on-line (or off-line) format that I can fill-in ? I added our names to FlyBase People today. Haruo Saito > Subject: PTP69D Reply to: PTP69D Dear Beverley, I missed 4E, 10D, etc, because I am used to think of them as DPTP rather than PTP, but obviously species designation is meaningless within the Drosophila data base. In any case, followings are the relevant information concerning PTP69D. I will follow the format as shown in the database. Gene symbol: Ptp69D. Synonym: DPTP, DPTP69D. Full name: protein tyrosine phosphatase 69D. Cytological map position: 69D by in situ hybridization. Function(s) of product: Immunoglobulin and fibronectin-type-III-repeat containing protein. Enzyme name/number: protein-tyrosine-phosphatase == EC 3.1.3.48. DNA AC no: M27699 (Genbank/EMBL). Protein AC no(s): PIR/B36182 and SWP/P16620. Reference FBrf0050872 reports the following: cDNA clone length: 4.8 Open reading frame size: 1462 aa Phenotypic information: PTP69D is a member of a family of receptor linked protein tyrosine phosphatases. Gene Reference(s): FBrf0050872. Thank you very much for your effort. -------------------------------------- To: Haruo Saito From: FlyBase Project Members Dear Dr. Saito, Thank you very much for your prompt response to my query. We will incorporate your communication in a future update. > Then there will be several other DPTP entries (DPTP4E, 10D, 64D <up>= original > DPTP</up>, and 99A that must be added. Actually there are gene entries in FlyBase for Ptp10D (FBgn0004370), Ptp4E (FBgn0004368), and Ptp99A (FBgn0010251). I'm not sure how you are accessing FlyBase data but a very easy way to get to these is to use the FlyBase WWW page (http://morgan.harvard.edu). Select FlyBase Data Access and then choose the SymbolSearch option. If you search for Ptp*, you will get all the genes I have mentioned. If you choose the genes option from the data access page you can search for tyrosine phosphatase to get a more extensive list of Drosophila genes encoding tyrosine phosphatases. Thanks in advance for any more corrections you may have. > I will supply the information about 64D. Is there > any on-line (or off-line) format that I can fill-in ? There is no form to fill out. Just send another note to flybase-updates. Thanks again. We really appreciate your help. Beverley Matthews for FlyBase